4.6 Article

Folate levels and N5,N10-methylenetetrahydrofolate reductase genotype (MTHFR) in mothers of offspring with neural tube defects:: A case-control study

Journal

ARCHIVES OF MEDICAL RESEARCH
Volume 32, Issue 4, Pages 277-282

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/S0188-4409(01)00292-2

Keywords

neural tube defects; folate levels; methylenetetrahydrofolate reductase; Mexico

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Background. Neural tube defects (NTDs) have been associated with biochemical factors involved in the conversion of homocysteine to methionine as folate deficiency and the mutation 677T in the N-5,N-10- methylenetetrahydrofolate reductase gene (MTHFR). Methods. A case-control study was performed to detect this mutation in 38 unrelated women with NTD deceased products and 31 mothers without antecedents of NTD offspring. All products were born in Nuevo Leon (northeastern Mexico) during 1997, Erythrocyte and plasmatic folate levels and the genotype of the 677 polymorphism at the MTHFR locus were analyzed in both groups. Results. Although no significant differences were found in mean blood folate levels, the percentage of women in the case group with erythrocyte folate levels <160 ng/mL was significantly higher than in the control group (75 vs. 51.2%, p <0.05). The proportion of women with plasma folate levels <3.5 ng/mL was higher in the case group (16.2 vs. 0%. p <0.01). Genotype analysis demonstrated a significantly higher proportion of 677T homozygous mothers with NTD products (39.6 vs, 9.1%. p <0.05). Allele frequencies for the 677T mutation were 0.55 and 0.36 for cases and controls, respectively. The odds ratio (OR) for having a NTD product was 6.1 (95%, CI 1.56-23.6) for homozygous 677T mothers vs, homozygous 677C and heterozygous mothers. Significantly low levels of erythrocyte folate were found in the 677C homozygous case group and in plasma folate in the 677C/677T heterozygous case mothers. Conclusions, Our study suggests that folate deficiency and MTHFR unfavorable genotype in mothers are important risk factors for severe NTD phenotype in our population. (C) 2001 IMSS. Published by Elsevier Science Inc.

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