Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 391, Issue 1, Pages 376-381Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.11.066
Keywords
NAMPT; Nicotinamide phosphoribosyltransferase; Hepatocytes; NMN biosynthesis; PBEF; Visfatin
Categories
Funding
- Deutsche Forschungsgemeinschaft [BE 1264/10-1]
- German Ministry of Science and Education Kompetenznetz Adipositas, BMBF, Berlin [FKZ 0313081F]
- Medical Faculty, University of Leipzig
- Human Tissue and Cell Research (HTCR)
Ask authors/readers for more resources
Circulating NAMPT (PBEF/visfatin) has pleiotropic functions and is secreted from adipocytes. Since it is doubtful whether serum levels can be explained by secretion from adipocytes alone, we asked whether hepatocytes are also able to liberate NAMPT. Using HepG2 cells and primary rat and human hepatocytes, release of NAMPT into the cell Culture Supernatant was found to Occur Constitutively in a time-dependent manner. In primary human hepatocytes, secretion within 24 h was far higher than the cellular content, but was neither influenced by inhibitors of secretion nor by glucose. insulin or TNF alpha. As determined by size exclusion chromatography. HepG2 lysates and supernatants primarily contained the dimeric form of NAMPT which exhibited similar in vitro specific enzymatic activity In primary human hepatocytes, secreted NAMPT was less active Our results demonstrate that human hepatocytes are a potential Source of circulating NAMPT (C) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available