Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 393, Issue 2, Pages 217-221Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.01.104
Keywords
Inner mitochondrial membrane; Ammonia transport; Yeast expression system; Aqua-ammoniaporin; Liver
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Funding
- Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR, Italy) [PRIN-Cofin 2004053441]
- Universita Italo-Francese
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 31670]
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, Argentina) [PIP 112 200801 01563]
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Aquaporin-8 (AQP8) is a membrane channel permeable to water and ammonia. As AQP8 is expressed in the inner mitochondrial membrane of several mammalian tissues, we studied the effect of the AQP8 expression on the mitochondrial transport of ammonia. Recombinant rat AQP8 was expressed in the yeast Saccharomyces cerevisiae. The presence of AQP8 in the inner membrane of yeast mitochondria was demonstrated by subcellular fractionation and immunoblotting analysis. The ammonia transport was determined in isolated mitochondria by stopped flow light scattering using formamide as ammonia analog. We found that the presence of AQP8 increased by threefold mitochondrial formamide transport. AQP8-facilitated mitochondrial formamide transport in rat native tissue was confirmed in liver (a mitochondrial AQP8-expressing tissue) vs. brain (a mitochondrial AQP8 non-expressing tissue). Comparative studies indicated that the AQP8-mediated mitochondrial movement of formamide was markedly higher than that of water. Together, our data suggest that ammonia diffusional transport is a major function for mitochondrial AQP8. (C) 2010 Elsevier Inc. All rights reserved.
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