Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 398, Issue 3, Pages 542-546Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.06.115
Keywords
Gold nanoparticle; Gene delivery system; Gene knockdown; shRNA
Categories
Funding
- Ministry of Education, Science and Technology [20100002201]
- Seoul RBD program [10543, 10550]
- Basic Research Promotion Fund (MOEHRD) [KRF-2008-331-000171]
- Priority Research Centers Program through the National Research Program of Korea [2009-0093817]
- Korea Foundation for International Cooperation of Science & Technology (KICOS) [2008-00656]
- Ministry of Education, Science & Technology (MoST), Republic of Korea [2008-00656] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2008-2000123, 2009-0093817, 2008-331-C00171] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The efficient delivery of nucleic acids into mammalian cells is a central aspect of research involving cell biology and medical applications, including the clinical treatment of genetic disorders. We report an efficient small hairpin RNA (shRNA) delivery system that utilizes a single species of gold nanoparticle-DNA oligonucleotide conjugate (AuNP-DNA oligo) as a universal carrier. In vitro synthesized shRNA that is specific to the p53 gene was efficiently delivered into HEK293 and HeLa human cell lines using an AuNP-DNA oligo. The delivery resulted in an 80-90% knockdown of p53 expression. The same AuNP-DNA oligo was also efficient for the delivery of another shRNA, which is specific to the Mcl-1 gene, as well as the repression of MCL-1 expression. The knockdown efficiency of shRNA that was delivered using an AuNP-DNA oligo was comparable with that of a liposome-based shRNA delivery method. Our results offer an alternate delivery system for shRNA that can be used on any gene of interest. (C) 2010 Elsevier Inc. All rights reserved.
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