Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 402, Issue 2, Pages 329-334Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.10.026
Keywords
Medial collateral ligament; bFGF; GDF-5; Hydrogel scaffold; Ligament healing
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Funding
- Japan Society for the Promotion of Science [20791040]
- Japanese Foundation for Research and Promotion of Endoscopy
- Ryobi Teien Memory Foundation
- Okayama Medical Foundation
- Ministry of Health, Labor and Welfare
- Grants-in-Aid for Scientific Research [20791040] Funding Source: KAKEN
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Basic fibroblast growth factor (bFGF) and growth and differentiation factor (GDF)-5 stimulate the healing of medial collateral ligament (MCL) injury. However, the effect of isolated and combined use of bFGF/GDF-5 remains still unclear. We investigated cellular proliferation and migration responding to bFGF/GDF-5 using rabbit MCL fibroblasts. Rabbit MCL injury was treated by bFGF and/or GDF-5 with peptide hydrogels. Gene expression and deposition of collagens in healing tissues were evaluated. bFGF/GDF-5 treatment additively enhanced cell proliferation and migration. bFGF/GDF-5 hydrogels stimulated Col1a1 expression without increasing Col3a1 expression. Combined use of bFGF/GDF-5 stimulated type I collagen deposition and the reorganization of fiber alignment, and induced better morphology of fibroblasts in healing MCLs. Our study indicates that combined use of bFGF/GDF-5 might enhance MCL healing by increasing proliferation and migration of MCL fibroblasts, and by regulating collagen synthesis and connective fiber alignment. (C) 2010 Elsevier Inc. All rights reserved.
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