Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 402, Issue 4, Pages 731-735Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.10.096
Keywords
Yeast; Yarrowia lipolytica; Fatty acid; Transcription
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Funding
- Ministry of Education Culture Sports Science and Technology of Japan
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The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes To identify a gene(s) regulating fatty acid utilization in Y lipolytica we first studied homologous genes to wOAF1 and PIP2 of Saccharomyces cerevisiae but their disruption did not change growth on oleic acid at all We next characterized a Y lipolytica gene POR1 (primary oleate regulator 1) an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans The deletion mutant of POR1 was defective in the growth on various fatty acids but not on glucose glycerol or n-hexadecane It exhibited slight defect on n-decane The transcriptional induction of genes Involved in beta-oxidation and peroxisome proliferation by oleate was distinctly diminished in the Delta por1 strains These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y,lipolytica (C) 2010 Elsevier Inc All rights reserved
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