Journal
PLANT SCIENCE
Volume 161, Issue 2, Pages 273-278Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-9452(01)00407-1
Keywords
Daucus carota; phytoalexin biosynthesis; signal transduction; GTP-binding protein; plasma membrane; oligogalacturonide elicitor
Categories
Ask authors/readers for more resources
Biosynthetic activity of carrot phytoalexin 6-methoxymellen was induced in cell suspension culture by the treatment with oligogalacturonide elicitor; however, the elicitor-induced activity appreciably reduced in the presence of suramin, a potent inhibitor of GTP-binding proteins. In contrast. addition of G-protein activators, such as mastoparan or GTP-gamma -S. to carrot cell culture triggered 6-methoxymellein production even in the absence of uronide elicitor. An appreciable GTPase activity was found in purified plasma membrane of cultured carrot cells, and the hydrolytic activity was significantly increased by the addition of elicitor. Carrot plasma membrane was capable of associating with GTP-gamma -S, and the binding ability was markedly increased in the presence of elicitor. However, the binding activity markedly decreased when the membrane preparation was pre-incubated with GTP but not with ATP. These observations strongly suggest that a certain GTP-binding protein located at plasma membrane of cultured carrot cells plays an important role in the oligogalacturonide elicitor-induced 6-methoxymellein production. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available