Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 390, Issue 3, Pages 684-688Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.10.028
Keywords
Bioluminescence; Biosynthesis; Cypridina luciferin; Luciferase; Mass spectrometry
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Coelenterazine is an imidazopyrazinone compound (3,7-dihydroimidazopyrazin-3-one structure) that is widely distributed in marine organisms and used as a luciferin for various bioluminescence reactions. We have used electrospray ionization-ion trap-mass spectrometry to investigate whether the deep-sea luminous copepod Metridia pacifica is able to synthesize coelenterazine. By feeding experiments using deuterium labeled amino acids of L-tyrosine and L-phenylalanine, we have shown that coelenterazine can be synthesized from two molecules of L-tyrosine and one molecule of L-phenylalanine in M. pacifica. This is the first demonstration that coelenterazine is biosynthesized from free L-amino acids in a marine organism. (C) 2009 Elsevier Inc. All rights reserved.
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