Journal
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
Volume 21, Issue 7, Pages 1244-1250Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/hq0701.092091
Keywords
atherosclerosis; lesion instability; time-resolved laser-induced fluorescence spectroscopy
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Funding
- NCRR NIH HHS [P41-RR01861] Funding Source: Medline
- NHLBI NIH HHS [R01 HL067377] Funding Source: Medline
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Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, II, (fatty streaks), III (preatheroma), IV (atheroma), V-a (fibrous), and V-b (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime tau (f); decay constants: tau (1) (fast-term), tau (2) (slow-term), A(1) (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. mie determined that a few intensity values at longer wavelengths (> 430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay), We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.
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