Journal
JOURNAL OF BONE AND MINERAL RESEARCH
Volume 16, Issue 7, Pages 1281-1290Publisher
WILEY
DOI: 10.1359/jbmr.2001.16.7.1281
Keywords
growth plate; chondrocytes; matrix vesicles; stromelysin-1; matrix metalloproteinase 3; latent transforming growth factor beta 1; transforming growth factor beta 1
Categories
Funding
- NIDCR NIH HHS [DE-08603, DE-05937] Funding Source: Medline
Ask authors/readers for more resources
Previous studies have shown that matrix vesicles isolated from cultures of costochondral growth zone chondrocytes and treated with 1 alpha ,25-dihydroxyvitamin D-3[1 alpha ;25(OH)(2)D-3] can activate recombinant human latent transforming growth factor beta1 (rhTGF-beta1). It is unknown what enzyme or other factor in the extracellular organelles is responsible for the activation. This study tested the hypothesis that enzymes present in matrix vesicles can activate latent TGF-beta1 and that this is regulated by 1 alpha ,25(OH)(2)D-3. To do this, we examined the ability of matrix vesicle extracts to activate small latent rhTGF-beta1. In addition, enzymes previously determined to be present in matrix vesicles were screened for their ability to activate small latent rhTGF-beta1. Recombinant human matrix metallo-proteinase 2 (rhMMP3; 72 kDa gelatinase), rhMMP3 (stromelysin 1), purified human plasminogen, and purified urokinase (plasminogen activator) were each tested at varying concentrations. To assess the role of cell maturation, we used a cell culture model in which chondrocytes are derived from two distinct zones of rat costochondral cartilage, the resting zone and the growth zone. Matrix vesicles were isolated from these cultures and then tested. The results showed that extracts of matrix vesicles produced by both growth zone and resting zone chondrocytes were able to activate small latent rhTGF-beta1. The effects were dose and time dependent, with greater activity being found in extracts of matrix vesicles from the growth zone chondrocyte cultures. Only rhMMP3 was able to activate small latent rhTGF-beta1, indicating that stromelysin-1, but not MMP-2, plasminogen, or urokinase, was involved. As observed in the extracts, the effect of rhMMP3 was time and dose dependent. When anti-MMP-3 antibody was added to matrix vesicle extracts from both cell types, activation of small latent rhTGF-beta1. was dose-dependently blocked. Neither 1 alpha ,25(OH)(2)D-3 nor 24R,25(OH)(2)D-3 had a direct effect on activation of small latent rhTGF-beta1 by the extracts. However, when intact matrix vesicles were treated with 1 alpha ,25(OH)(2)D-3, their ability to activate small latent rhTGF-beta1 was increased. Inhibition of phospholipase A,with quinacrine blocked the la,25(OH),D,dependent effect. These results suggest that the ability of 1 alpha ,25(OH)(2)D-3-treated matrix vesicles to activate small latent TGF-PI is via action of the secosteroid on the matrix vesicle membrane, not on the enzymes responsible for activating latent TGF-beta1. Because matrix vesicles isolated from growth zone chondrocytes have been shown to contain increased phospholipase A(2) activity after treatment with la;25(OH),D,, it is likely that this secosteroid promotes loss of membrane integrity through phospholipase A(2)-dependent formation of lysophospholipids, resulting in the release of MMP3 into the matrix, where latent TGF-beta1 is stored. Taken together, the results of the current study show that matrix vesicles produced by growth plate chondrocytes contain MMP-3, that this enzyme is at least partially responsible for activation of small latent TGF-beta1 in the matrix, and that 1 alpha ,25(OH)(2)D-3 regulates MMP release from matrix vesicles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available