Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 378, Issue 2, Pages 264-268Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.11.047
Keywords
Autotaxin; LPS; THP-1 cells; PKR; JNK; p38 MAPK; LPA
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Funding
- National High Technology Research and Development Program [2006AA02Z4A6]
- Science and Technology Program of Beijing Municipality [Z0006329000191]
- National Nature Science Foundation of China [30570409, 30770030]
- Beijing NOVA Program [2005B47]
- Program for New Century Excellent Talents in University [NCET-05-0145]
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Autotaxin (ATX) is a secreted enzyme with lysophospholipase D (lysoPLD) activity, which converts lysophosphatidylcholine(LPC) into lysophosphatidic acid (LPA), a bioactive phospholipid involved ill numerous biological activities, including cell proliferation, differentiation, and migration. In the present study, we found that bacterial lipopolysaccharide(LPS), a well-known initiator of the inflammatory response, induced ATX expression in monocytic THP-1 cells. The activation of PKR, JNK, and p38 MAPK was required for the ATX induction. The LPS-induced ATX ill THP-1 cells was characterized as the beta isoform. In the presence of LPC, ATX could promote the migrations of THP-1 and Jurkat cells, which was inhibited by pertussis toxin (PTX), an inhibitor of Gi-mediated LPA receptor signaling, In summary, LPS induces ATX expression ill THP-1 cells via a PKR, JNK and p38 MAPK-mediated mechanism, and the ATX induction is likely to enhance immune cell migration in proinflammatory response by regulating LPA levels in the microenvironment. (C) 2008 Elsevier Inc. All rights reserved.
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