Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 378, Issue 3, Pages 478-482Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.11.066
Keywords
Dynamin; Phagocytosis; Endocytosis; Testis; Actin dynamics
Categories
Funding
- Ministry of Education, Science, Sports and Culture of Japan
- Okayama Medical Foundation
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Dynamin 2 has been reported to be implicated in phagocytosis. However, the mode of action of dynamin is Poorly understood. In this Study, we examined whether dynamin 2 participates in actin assembly during phagocytosis in Sertoli cells. In the presence of dynasore, a dynamin inhibitor, phagocytosis was reduced by 60-70% in Sertoli cells and macrophages. Scanning electron microscopy revealed that Sertoli cells treated with dynasore were unable to form phagocytic cups. In addition, dysfunction of dynamin 2 reduced both actin polymerization and recruitment of actin and dynamin 2 to phosphatidylinositol (4,5) bisphosphate [ PI(4,5)P-2]-containing liposomes. The formation of dynamin 2-positive ruffles of Sertoli cells was decreased by 60-70% by sequestering PI(4,5)P2 either by expression of PH domain of PLC delta or treatment with neomycin. These results strongly Suggest that dynamin 2 is involved in actin dynamics and the formation of dynamin 2-positive ruffles during phagocytosis. (C) 2008 Elsevier Inc. All rights reserved.
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