Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 379, Issue 2, Pages 489-493Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.12.097
Keywords
RNA polymerase III; tRNA; Ribosomal proteins; Saccharomyces cerevisae; TFIIIE
Categories
Funding
- Ministry of University and Research
- Fondazione Cariparma
Ask authors/readers for more resources
A yeast nuclear fraction of unknown coin posit ion, named THIIIE, was reported previously to enhance transcription of tRNA and 5S rRNA genes in vitro. We show that TFIIIE activity Co-purifies with a specific subset of ribosomal proteins (RPs)which, as revealed by chromatin immunoprecipitation analysis, generally interact with tRNA and 5S rRNA genes, but not with a Pol II-specific promoter. Only Rp16Ap and Rp16Bp, among the tested RPs, were found associated to a TATA-containing tRNA (IIc)(TAT) gene. The RPL6A gene also emerged as a Strong multicopy suppressor of a conditional mutation in the basal transcription factor TFIIIC, while RPL26A and RoI 14A behaved as weak suppressors. The data delineate a novel extra-ribosomal role for one or a few RPs which, by influencing 5S rRNA and tRNA synthesis, Could play a key role in the coordinate regulation of (lie different sub-pathways required for ribosome biogenesis and functionality. (C) 2008 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available