Journal
INFECTION AND IMMUNITY
Volume 69, Issue 7, Pages 4647-4653Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.7.4647-4653.2001
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Streptococcus intermedius is associated with deep-seated purulent infections. In this study, we investigated expression and functional activities of antigen VII in S, intermedius, The S, intermedius antigen I/II appeared to be cell surface associated, with a molecular mass of approximately 160 kDa, Northern blotting indicated that the S, intermedius NCTC 11324 antigen VII gene was transcribed as a monocistronic message. Maximum expression was seen during the early exponential phase, Insertional inactivation of the antigen I/II gene resulted in reduced hydrophobicity during early exponential phase, whereas no effect was detected during mid- and late exponential phases. Binding to human fibronectin and laminin was reduced in the isogenic mutant, whereas binding to human collagen types I and IV and to rat collagen type I was not significant for either the wild type or the mutant. Compared to the wild type, the capacity of the isogenic mutant to induce interleukin 8 (IL-8) release by THP-1 monocytic cells was significantly reduced. The results indicate that the S. intermedius antigen I/II is involved in adhesion to human receptors and in IL-8 induction.
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