Journal
BIOTECHNOLOGY LETTERS
Volume 23, Issue 14, Pages 1171-1174Publisher
SPRINGER
DOI: 10.1023/A:1010560911400
Keywords
glycidyl methacrylate; immobilization; industrial; invertase; periodate
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Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g(-1), with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h(-1). The obtained specific productivity at 40 degreesC of 3 kg l(-1) h(-1) is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 degreesC and at a flow rate of 3 bed volumes h(-1).
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