Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 389, Issue 3, Pages 443-448Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.08.162
Keywords
CpG-DNA; hBD-2; NF-kappa B; Lymphocytes; B cells
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Funding
- Ministry for Health, Welfare & Family Affairs, Republic of Korea [A084589]
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Defensins have a broad range of antimicrobial activity against bacteria, fungi, and viruses. The expression of human beta-defensin-2 (hBD-2) is prevalently observed in epithelial cells and is induced by bacterial infection. Here, we have shown that the expression of the hBD-2 gene and release of hBD-2 protein into the medium is up-regulated in response to CpG-DNA in human B cell line RPMI 8226. The induction of hBD-2 was dependent on CG sequence and phosphorothioate backbone-modification. This was also confirmed in primary human lymphocytes. To shed light on the molecular mechanism involved in hBD-2 induction by CpG-DNA, we examined the contribution of the NF-kappa B signaling pathway in RPMI 8226 cells. Suppression of MyD88 function and inhibition of NF-kappa B nuclear localization blocked hBD-2 induction. The NF-kappa B pathway inhibitors also abolished hBD-2 induction. These results may contribute to a better understanding on the therapeutic effects of CpG-DNA against infectious diseases. (C) 2009 Elsevier Inc. All rights reserved.
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