Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 390, Issue 3, Pages 659-661Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.10.023
Keywords
Antioxidant capacity; Blood plasma; Antioxidants; Peroxynitrite; Hypochlorite
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Peroxynitrite and hypochlorite are oxidants relevant in many pathological situations. We propose a simple spectrophotometric assay to determine antioxidant capacity against hypochlorite and peroxynitrite based on protection against Pyrogallol Red decolorization. The assay can be performed on a microplate and requires minute amounts of material. Standard antioxidants show different reactivities for both oxidants. Antioxidant capacity of blood plasma (anticoagulated with EDTA) of healthy persons was found to be 559 +/- 49 mu mol/l and 11.6 +/- 1.2 mmol/l of ascorbic acid equivalents for peroxynitrite and hypochlorite, respectively. (C) 2009 Elsevier Inc. All rights reserved.
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