Journal
INFECTION AND IMMUNITY
Volume 69, Issue 7, Pages 4698-4701Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.7.4698-4701.2001
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Di-, tri-, and tetrasaccharides, synthesized according to the chemical structure of pneumococcal polysaccharide type 3 (PS3), were coupled to the cross-reactive material (CRM197) of modified diphtheria toxin in different molar carbohydrate/protein ratios using the squarate coupling method. To study protective immunity, female BALB/c mice were subcutaneously immunized twice (with a 3-week interval) using the amount of conjugates corresponding to 2.5 mug of oligosaccharide per mouse. The conjugates evoked PS3 binding immunoglobulin G antibodies that lasted for at least 7 weeks after the booster. Immunogenicity was not influenced by the carbohydrate/protein ratio. All mice with PS3-specific antibodies survived the intraperitoneal challenge with Streptococcus pneumoniae type 3, Therefore, synthetic oligosaccharide-protein conjugates might have potential as vaccines.
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