Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 382, Issue 3, Pages 637-641Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.03.091
Keywords
Abiotic stress; Antiporter; Arabidopsis thaliana; AtNHX4; Salt tolerance
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Funding
- National Basic Research Program of China [2006CB100106]
- National Natural Science Foundation of China [30571196, 0933ZF11C1, 0933Z411C1]
- Ministry of Science and Technology of China [2007AA10Z187]
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AtNHX4 belongs to the monovalent cation:proton antiporter-1 (CPA1) family in Arabidopsis. Several members of this family have been shown to be critical for plant responses to abiotic stress, but little is known on the biological functions of AtNHX4. Here, we provide the evidence that AtNHX4 plays important roles in Arabidopsis responses to salt stress. Expression of AtNHX4 was responsive to salt stress and abscisic acid. Experiments with CFP-AtNHX4 fusion protein indicated that AtNHX4 is vacuolar localized. The nhx4 mutant showed enhanced tolerance to salt stress, and lower Na+ content under high NaCl stress compared with wild-type plants. Furthermore, heterologous expression of AtNHX4 in Escherichia coli BL21 rendered the transformants hypersensitive to NaCl. Deletion of the hydrophilic C-terminus of AtNHX4 dramatically increased the hypersensitivity of transformants, indicating that AtNHX4 may function in Na+ homeostasis in plant cell, and its C-terminus plays a role in regulating the AtNHX4 activity. (C) 2009 Elsevier Inc. All rights reserved
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