Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 384, Issue 4, Pages 530-534Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.05.014
Keywords
Glycine transporter; Membrane rafts; Fluorescence resonance energy transfer; Fluorescence life time imaging; Cholesterol; Co-localization; Glycine transport; GLYT1
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Funding
- The University of Sydney
- NHMRC [402564]
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Membrane rafts, the highly-ordered, cholesterol-rich microdomains of the plasma membrane play important roles in cellular functions. In this study, GLYT1-CFP and GLYT2-CFP were constructed, followed by investigation of whether the tagged transporters associate with a fluorescence probe that labels membrane rafts (DiIC16) by using Fluorescence Resonance Energy Transfer. A close association was observed between DiIC16 and GLYT1-CFP, but not for GLYT2-CFP. The glycine transport ability of GLYT1 is also highly dependent on the integrity of this area. Together, the results suggest that GLYT1 and membrane rafts are co-localized in the membrane, and that this influences the rate of glycine transport. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.
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