Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 376, Issue 4, Pages 633-636Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.08.147
Keywords
Click chemistry; Immobilization; Peptide nucleic acid; DNA chip
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Funding
- Ministry of Science and Technology, Daejeon Metropolitan City
- Korean Government (MOEHRD) [KRF-2006-331-D00114]
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The fluorogenic 1,3-Huisgen dipolar cycloaddition reaction was used as part of a novel immobilization strategy of PNA capture probes on a microarray. By using this click chemistry, azidocoumarin-anchored PNA probes were immobilized on phenyl acetylene-modified glass slides with the simultaneous generation of the fluorescent triazolycoumarin moiety. Since the emitting moieties are generated in the immobilization reaction itself, fluorescent signals can be used to directly monitor the integrity of immobilization in a nondestructive manner. By using this strategy, PNA microarrays were prepared and successfully employed to perform microarray-based diagnosis of selected Mutations in the breast cancer susceptibility gene BRCA1. (C) 2008 Elsevier Inc. All rights reserved.
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