Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 39, Issue 7, Pages 2648-2651Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.39.7.2648-2651.2001
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Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA, The resistant and susceptible colonies of each of the heterogeneous H, pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes, In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary W, pylori population is used to test for clarithromycin susceptibility.
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