Journal
BIOCHIMIE
Volume 83, Issue 7, Pages 653-658Publisher
EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/S0300-9084(01)01302-5
Keywords
carbohydrate-lectin binding; Sd(a) determinant; surface plasmon resonance; Tamm-Horsfall glycoprotein
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The binding properties of a spacer-linked synthetic Sd(a) tetrasaccharide beta -D-GalpNAc-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (1), two tetrasaccharide mimics beta -D-Galp-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5) -NH2 (2) and beta -D-GlcpNAc-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (3), and two trisaccharide mimics beta -D-GalpNAc-(1-->4)-3-O-(SO3H)-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (4) and beta -D-GalpNAc-(1-->4)-3-O-(CH2COOH)-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (5) with lectins from Dolichos biflorus (DBL), Maackia amurensis (MAL), Phaseolus limensis (PLL), Ptilota plumosa (PPL), Ricinus communis 120 (RCL120) and Triticum vulgaris (wheat germ agglutinin, WGA) have been investigated by surface plasmon resonance (SPR) detection. MAL, PPL, RCL120 and WGA did not display any binding activity with compounds 1-5. However, DBL and PLL, both exhibiting GalNAc-specificity, showed strong binding activity with compounds 1, 4 and 5, and 1, 3, 4 and 5, respectively. The results demonstrate that SPR is a very useful analysis system for identifying biologically relevant oligosaccharide mimics of the Sd a determinant. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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