Journal
METHODS
Volume 24, Issue 3, Pages 297-306Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/meth.2001.1190
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Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system. (C) 2001 Academic Press.
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