Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 365, Issue 1, Pages 82-88Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2007.10.128
Keywords
opioid receptors; glycosylation; N-glycans; lipid rafts; brain
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Funding
- NIDA NIH HHS [R01 DA017302-03, P30 DA013429-099002, P30 DA013429, R01 DA017302, R01 DA17302, P30 DA13429, R01 DA017302-04] Funding Source: Medline
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The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by Western blot with a polyclonal antibody against a C-terminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr's) of 75 kDa (CPu) vs. 66 kDa (thalamus) for the rat, and 74 kDa (CPu) vs. 63 kDa (thalamus) for the mouse, which was due to its differential N-glycosylation. Rat MOR in CPu was found mainly associated with low-density cholesterol- and ganglioside M1 (GM1)enriched membrane subdomains (lipid rafts), while the MOR in the thalamus was present in rafts and non-rafts without preference. Cholesterol reduction by methyl-beta-cyclodextrin decreased DAGMO-induced [S-35]GTP gamma S binding in rat CPu membranes to a greater extent than in the thalamus membranes. (c) 2007 Elsevier Inc. All rights reserved.
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