Journal
BRAIN RESEARCH PROTOCOLS
Volume 7, Issue 3, Pages 235-240Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1385-299X(01)00069-1
Keywords
spinal cord; excitatory postsynaptic currents (EPSCs); inhibitory postsynaptic currents (IPSCs); substantia gelatinosa; pain; rat
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We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (A delta and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root A delta fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice. (C) 2001 Elsevier Science B.V. All rights reserved.
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