Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 377, Issue 4, Pages 1179-1184Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.10.111
Keywords
Circadian rhythms; Co-culture; Gene expression; SCN; Slice culture
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Funding
- National Natural Science Foundation of China [30400148, 30430280, 3042801040, 30371574]
- Chinese National Scientific Technology Research Funds [2006CB500701]
- National Basic Research Program of China [2006CB 0F 0603]
- Beijing Municipal Science & Technology Commission [2007CB947700]
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The mammalian circadian pacemaker has been localized to the hypothalamic suprachiasmatic nucleus (SCN), where a set of clock genes and their resulting proteins form interlocking transcriptional/translational feedback loops to sustain molecular and functional oscillations. Interestingly, peripheral tissues and stimulated fibroblasts have also displayed daily oscillations, which are thought to be synchronized by the SCN in vivo. However, intercellular communications between the SCN and other tissues or cells remain poorly understood. Therefore, a novel co-culture model was established in the present Study to Understand the interactions between central and peripheral oscillators in co-cultures of SCN slices and NIH/3T3 cells in a serum-free condition. Expression profiles of Per1 and Rev-Erb alpha were measured in NIH/3T3 cells using real-time PCR. Results demonstrated that diffusible signals released from SCN slices could regulate molecular rhythms in Cultured fibroblasts. Moreover, Rev-Erb alpha oscillation was more robust and appeared earlier than Per1. (C) 2008 Elsevier Inc. All rights reserved.
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