Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 368, Issue 3, Pages 593-599Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.01.127
Keywords
Ca2+ signaling; endoplasmic reticulum
Categories
Funding
- NINDS NIH HHS [R01 NS046783, R01 NS046783-01A1, NS046783] Funding Source: Medline
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The hepatitis C non-structural protein 5A (NS5A) is a Zn2+-binding phosphoprotein essential for viral replication. Expression of NS5A perturbs intracellular Ca2+ levels by an undefined mechanism, activating transcription factors implicated in the chronic pathogenesis of hepatitis infections. Here, we demonstrate that regulated expression of NS5A enhanced the passive leak of Ca2+ from a subset of the endoplasmic reticulum (ER) Ca2+ stores. This action was not replicated by expression of the amphipathic NH2-membrane anchoring domain of NS5A alone, despite targeting to intracellular membranes. Depletion of the NS5A-targeted ER Ca2+ store was prevented under conditions of ample ATP supply suggesting compensatory Ca2+ ATPase activity, but observed under conditions of ATP insufficiency and in intact cells expressing NS5A. (c) 2008 Elsevier Inc. All rights reserved.
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