Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 374, Issue 3, Pages 454-459Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.07.041
Keywords
Arabidopsis; glucokinase; glucose sensing; Synechocystis
Categories
Funding
- Crop Functional Genomics Center [CG2151]
- KOSEF [R01-2007-000-20149-0]
- Ministry of Science and Technology, Korea
- National Research Foundation of Korea [R01-2007-000-20149-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Unlike Arabidopsis hexokinase (AtHXK) 1, cyanobacterial glucokinase (cGlk, Sll0593) from Synechocystis sp. PCC6803 does not function endogenously as a glucose sensor for glucose repression of photosynthesis-related genes such as psbA2, psbD2, rbcS, and rbcL. However, when cGlk was constitutively expressed in the cytosol of the glucose insensitive AtHXK 1 null mutant gin2-1, transgenic plants showed glucose sensitive phenotypes similar to those of wild type plants, namely glucose-induced decreases in Chl content and transcript levels of genes encoding Chl binding proteins (CAB1) and Rubisco small subunit (RBCS). Therefore, we suggest that cGlk's ability to complement glucose sensing activity in higher plants is attributable to the presence of cGlk-interacting proteins present in Arabidopsis, but absent in Synechocystis. (C) 2008 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available