Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 372, Issue 4, Pages 752-755Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.05.100
Keywords
acid-sensing ion channels; channel assembly; subunit stoichiometry; atomic force microscopy
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Funding
- Biotechnology and Biological Sciences Research Council [BB/D015545/1] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/D015545/1] Funding Source: Medline
- BBSRC [BB/D015545/1] Funding Source: UKRI
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There has been confusion about the subunit stoichiometry of the degenerin family of ion channels. Recently, a crystal structure of acid-sensing ion channel (ASIC) 1a revealed that it assembles as a trimer. Here, we used atomic force microscopy (AFM) to image unprocessed ASIC1 a bound to mica. We detected a mixture of subunit monomers, dimers and trimers. In some cases, triple-subunit clusters were clearly visible, confirming the trimeric structure of the channel, and indicating that the trimer sometimes disaggregated after adhesion to the mica surface. This AFM-based technique will now enable us to determine the subunit arrangement within heteromeric ASICs. (c) 2008 Elsevier Inc. All rights reserved.
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