Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 285, Issue 1, Pages 58-63Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/bbrc.2001.5123
Keywords
Alzheimer's disease; amyloid; A beta; fluorescein; time-resolved fluorescence anisotropy
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Time-resolved anisotropy measurements (TRAMS) have been used to study the aggregation of the beta -amyloid (A beta) peptide which is suspected of playing a central role in the pathogenesis of Alzheimer's Disease (AD), The experiments, which employ small quantities of fluorescently-labelled A beta, in addition to the untagged peptide, have shown that the sensitive TRAMS technique detects the presence of preformed seed particles in freshly prepared solutions of A beta, More importantly, as 100 muM solutions of A beta containing tagged A beta at a concentration level of either 0.5 or 1 muM are incubated, the TRAMS prove capable of detection of the peptide aggregation process through the appearance of a continuously increasing residual anisotropy within the time-resolved fluorescence data. The method detects A beta aggregation in its earliest stages, well before complexation becomes apparent in more conventional methods such as the thioflavin T fluorescence assay. The TRAMS approach promises to provide a most attractive route for establishment of a high-throughput procedure for the early detection of the presence of amyloid aggregates in the screening of biological samples, (C) 2001 Academic Press.
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