☆ 4.1 Article

Recombinant lipase from Candida rugosa for regioselective hydrolysis of peracetylated nucleosides. A comparison with commercial non-recombinant lipases

BIOCATALYSIS AND BIOTRANSFORMATION (2010)

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Journal

BIOCATALYSIS AND BIOTRANSFORMATION
Volume 28, Issue 2, Pages 108-116

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.3109/10242420903497362

Keywords

Lipase; immobilization; nucleosides; regioselectivity

Categories

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology

Funding

  1. BIOCAT REGINS [2E0006R]

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Commercial lipases from the yeast Candida rugosa have been compared with two recombinant C. rugosa lipases, rCRL1 and rCRL1lid3, with respect to their immobilization and exploitation in biotransformations aimed at the synthesis of pyrimidine nucleosides. Immobilization on octyl-agarose and decaoctyl-Sepabeads but not on Eupergit

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