Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 26, Issue 5, Pages 444-449Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420802323458
Keywords
Bacillus pumilus; bioconversion; omega-hydroxy oleic acid; mutagenesis
Funding
- K. C. Wong Education Foundation
- Hong Kong in Ningbo University
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Chemical mutation of a strain producing hydroxy-fatty acid from oleic acid (OA) using NTG (N-methyl-N'-nitro-N-nitrosoguanidine) resulted in a high percentage of improved mutants. A positive screening procedure yielded several high producers, specifically the strain Bacillus pumilus M-F641 (BP M-F641). This strain produced predominantly omega(-1)-hydroxy fatty acid and could utilize higher concentrations of OA than the parent strain. In shake flask culture, the best omega(-1)-hydroxy fatty acid concentration and yield (the ratio of omega(-1)-hydroxy fatty acid accumulation to OA consumption) reached 570 mg L-1 and 11.5%, respectively. Repeated tests showed that the mutant BP M-F641 was genetically stable.
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