Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 26, Issue 1-2, Pages 152-160Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420701804541
Keywords
glycogen; macrophage; nitric oxide; TNF-alpha; immunostimulating activity; molecular structure
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Glycogen is exclusively known as an energy and carbon reserve in animal cells and micro-organisms. We synthesized glycogens of varying molecular weight by using three enzymes, and investigated the relationship between the structure and immunostimulating activity of glycogen. These results indicated that glycogens with a molecular weight of more than 1.0 x 10(7) hardly activated RAW264.7, a murine macrophage cell line, whereas glycogens of 5.0-6.5 x 10(6) strongly stimulated RAW264.7 in the presence of interferon-, leading to augmented production of nitric oxide, tumour necrosis factor- and interleukin-6. Additionally, the number-average unit chain length and the exterior and interior chain lengths of the glycogens showed a minor correlation between active and less-active glycogen derivatives. On the other hand, the binding activity of glycogen toward RAW264.7 did not depend on the molecular weight of glycogen. The available evidence suggests that the macrophage-stimulating activity of glycogen is strictly related to its molecular weight rather than to fine structural properties.
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