Journal
JOURNAL OF BIOTECHNOLOGY
Volume 88, Issue 3, Pages 183-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(01)00281-4
Keywords
coronaviruses; viral vectors; transcription regulatory sequences; virus tropism
Categories
Ask authors/readers for more resources
Both helper dependent expression systems, based on two components, and single genomes constructed by targeted recombination, or by using infectious cDNA clones, have been developed. The sequences that regulate transcription have been characterized mainly using helper dependent expression systems and it will now be possible to validate them using single genomes. The genome of coronaviruses has been engineered by modification of the infectious cDNA leading to an efficient (> 20 mug ml(-1)) and stable (> 20 passages) expression of the foreign gene. The possibility of engineering the tissue and species tropism to target expression to different organs and animal species, including humans, increases the potential of coronaviruses as vectors. Thus, coronaviruses are promising virus vectors for vaccine development and, possibly, for gene therapy. (C) 2001 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available