Journal
ANALYST
Volume 140, Issue 22, Pages 7578-7585Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an01588j
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Funding
- Chinese 973 Project [2012CB933302]
- National Natural Science Foundation of China [21175022]
- Ministry of Science & Technology of China [2012AA022703, 2015AA020502]
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The developed method for monitoring GST, an important drug metabolic enzyme, could greatly facilitate researches on relative biological fields. In this work, we have developed a SERS technique to monitor the absorbance behaviour of 6-mercaptopurine (6-MP) and its glutathione-S-transferase (GST)-accelerated glutathione (GSH)-triggered release behaviour on the surface of gold nanoflowers (GNFs), using the GNFs as excellent SERS substrates. The SERS signal was used as an indicator of absorbance or release of 6-MP on the gold surface. We found that GST can accelerate GSH-triggered release behaviour of 6-MP from the gold surface. We speculated that GST catalyzes nucleophilic GSH to competitively bind with the electrophilic substance 6-MP. Experimental results have proved that the presented SERS protocol can be utilized as an effective tool for accessing the release of anticancer drugs.
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