Journal
EMBO JOURNAL
Volume 20, Issue 14, Pages 3821-3830Publisher
WILEY
DOI: 10.1093/emboj/20.14.3821
Keywords
alternative splicing; CD44; exonic splicing enhancer; pre-mRNA; YB-1
Categories
Funding
- NHLBI NIH HHS [R01 HL45565, R01 HL045565] Funding Source: Medline
- NIGMS NIH HHS [GM38526, R01 GM038526] Funding Source: Medline
Ask authors/readers for more resources
Exon enhancers are accessory pre-mRNA splicing signals that stimulate exon splicing. One class of proteins, the serine-arginine-rich (SR) proteins, have been demonstrated to bind enhancers and activate splicing. Here we report that A/C-rich exon enhancers (ACE elements) are recognized by the human YB-1 protein, a non-SR protein. Sequence-specific binding of YB-1 was observed both to an ACE derived from an in vivo iterative selection protocol and to ACE elements in an alternative exon (v4) from the human CD44 gene. The ACE element that was the predominant YB-1 binding site in CD44 exon v4 was required for maximal in vivo splicing and in vitro spliceosome assembly. Expression of wild-type YB-1 increased inclusion of exon v4, whereas a truncated form of YB-1 did not. Stimulation of exon v4 inclusion by wild-type YB-1 required the ACE necessary for YB-1 binding in vitro, suggesting that YB-1 stimulated exon inclusion in vivo by binding to an exonic ACE element. These observations identify a protein in addition to SR proteins that participates in the recognition of exon enhancers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available