Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 923, Issue 1-2, Pages 215-227Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)00982-7
Keywords
electrochromatography; monolithic polymer column; porosity; micropore
Ask authors/readers for more resources
A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 mum I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column proper-ties, such as morphology, porosity, stability and reproducibility, were extensively tested. (C) 2001 Elsevier Science B.V. rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available