Journal
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
Volume 467, Issue -, Pages 1308-1311Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-9002(01)00648-9
Keywords
X-ray microscopy; computed tomography; cryo techniques
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Using the photoelectric absorption contrast between water and protein at 2.4 nm wavelength, cryo X-ray microscopy has visualized protein structures down to 30 mn size in unstained, unsectioned biological specimens. Due to the large depth of focus of the Fresnel zone plate objectives, computed tomography based on a tilt series of X-ray microscopic images can be used to reconstruct the three-dimensional specimen structure. This method has been applied to the green alga Chlamydomonas reinhardtii, and to cell nuclei of male Drosophila melanogaster fruit fly cells. (C) 2001 Elsevier Science B.V. All rights reserved.
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