Journal
CELL
Volume 106, Issue 2, Pages 233-241Publisher
CELL PRESS
DOI: 10.1016/S0092-8674(01)00435-4
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Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 Angstrom resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position + 13 to + 15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.
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