Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 924, Issue 1-2, Pages 43-52Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)00990-6
Keywords
mathematical modelling; capillary electrophoresis; polymers; DNA
Funding
- NIGMS NIH HHS [5-T32 GM 08449-06] Funding Source: Medline
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We recently demonstrated that the molecular mass distribution of an uncharged polymer sample can be analyzed using free-solution capillary electrophoresis of DNA-polymer conjugates. In these conjugates, the DNA is providing the electromotive force while the uncharged polydisperse polymer chains of the sample retard the DNA engine with different amounts of hydrodynamic drag. Here we present a theoretical model of this new analytical method. We show that for the most favourable, diffusion-limited electrophoresis conditions, there is actually an optimal DNA size to achieve the separation of a given polymer sample. Moreover, we demonstrate that the effective friction coefficient of the polymer chains is related to the stiffness of the two polymers of the conjugate, thus offering a method to estimate the persistence length of the uncharged polymer through mobility measurements. Finally, we compare some of our predictions with available experimental results. (C) 2001 Elsevier Science B.V. All rights reserved.
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