Journal
LIFE SCIENCES
Volume 69, Issue 10, Pages 1213-1222Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0024-3205(01)01218-8
Keywords
virgin olive oil; phenolic antioxidants; nitric oxide; peroxynitrite; nitrergic neurotransmission; anococcygeus muscle
Ask authors/readers for more resources
The major phenolics from the polar fraction of virgin olive oil (caffeic acid, oleuropein, tyrosol and hydroxytyrosol) have well-established antioxidant activities but their effects on reactive nitrogen species and nitrergic neurotransmission have not been fully investigated. The three catechol compounds were active as scavengers of nitric oxide generated spontaneously from the decomposition of sodium nitroprusside (approximate to 50% inhibition achieved at 75 muM), and had similar ability to scavenge chemically generated peroxynitrite, as determined by an alpha (1)-antiproteinase inactivation assay (67.2 %-92.4 % reduction when added at 1mM). Tyrosol was less active in these tests, but does not possess the catechol functionality. Despite their ability to interact with chemically prepared nitric oxide, neither oleuropein nor hydroxytyrosol at 5 muM altered NO. -mediated relaxations of the nerve-stimulated rat anococcygeus preparation, but this may be because the nitrergic transmitter is protected from the effects of externally applied scavengers. In conclusion, the phenolics found in virgin olive oil possess ability to scavenge reactive oxygen and nitrogen species that are implicated in human pathologies, but their impact may be restricted to those species present in the extracellular environment. (C) 2001 Elsevier Science Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available