Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 98, Issue 16, Pages 9431-9436Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.161099898
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- NIDDK NIH HHS [R01 DK048823, P30 DK034989, R37 DK025636, R01 DK025636] Funding Source: Medline
- NIEHS NIH HHS [P30 ES001247, R01 ES006484, P30 ES003828] Funding Source: Medline
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Uptake of organic solutes and xenobiotics by mammalian cells is mediated by ATP-independent transporters, and four families of transporters have now been identified. To search for novel organic solute transporters, a liver cDNA library from an evolutionarily primitive marine vertebrate, the little skate Raja erinacea, was screened for taurocholate transport activity by using Xenopus laevis oocytes. In contrast to the organic anion transporters identified to date, a transport activity was identified in this library that required the coexpression of two distinct gene products, termed organic solute transporter alpha and beta (Ost alpha, Ost beta). Ost alpha cDNA encodes for a protein of 352 as and seven putative transmembrane (TM) domains. Ost beta contains 182 as and has at least one and perhaps two TM domains. There is no significant sequence identity between Ost alpha and Ost beta, and only low identity with sequences in the databases; however, Ost alpha bears a resemblance to some G protein-coupled receptors, and Ost beta exhibits 22% amino acid identity with the C-terminal TM and intracellular domains of protocadherin-gamma, a cell surface glycoprotein. Xenopus oocytes injected with the cRNA for both Osta and Ost beta, but not each separately, were able to take up taurocholate, estrone sulfate, digoxin, and prostaglandin E-2, but not p-aminohippurate or S-dinitrophenyl glutathione. Transport was sodium-independent, saturable, and inhibited by organic anions and steroids, including the major skate bile salt, scymnol sulfate. These results identify an organic anion transporter composed of a putative seven-helix TM protein and an ancillary membrane polypeptide.
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