Journal
CRYOBIOLOGY
Volume 43, Issue 1, Pages 32-45Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/cryo.2001.2338
Keywords
cryoprotectants; freeze tolerance; wood frog
Categories
Ask authors/readers for more resources
To determine whether episodes of natural freezing and thawing altered the metabolic makeup of wood frog (Rana sylvatica) organs, the maximal activities of 28 enzymes of intermediary metabolism were assessed in six organs (brain, heart, kidney, liver, skeletal muscle, gut) of control (5degreesC acclimated), frozen (24 h at -3degreesC), and thawed (24 It back at 5degreesC) frogs. The enzymes assessed represented pathways including glycolysis, gluconeogenesis, amino acid metabolism. fatty acid metabolism, the TCA cycle, and adenylate metabolism. Organ-specific responses seen included (a) the number of enzymes affected by freeze-thaw (I in gut ranging to 17 in heat), (b) the magnitude and direction of response (most Often enzyme activities decreased during freezing and rebounded with thawing but, liver showed freeze-specific increases in several enzymes), and (c) the response to freezing versus thawing (enzyme activities in gut and kidney changed during freezing, whereas most enzymes in skeletal muscle responded to thawing), Overall. the data show that freeze-thaw implements selected changes to the maximal activities of various enzymes of intermediary metabolism and that these may aid organ-specific responses that alter fuel use during freeze-thaw, support cryoprotectant metabolism, and aid organ endurance of freeze-induced ischemia. (C) 2001 Elsevier Science.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available