Journal
JOURNAL OF DAIRY RESEARCH
Volume 68, Issue 3, Pages 429-436Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0022029901004940
Keywords
PCR cytochrome b; milk; animal identification
Ask authors/readers for more resources
Specific PCR, amplifying a fragment of the mitochondrially encoded cytochrome b gene, are developed for discrimination of chicken, turkey, pig, cow and sheep. these PCR tests could be applied for detection and discrimination of animal food ingredients. For origin identification of milk, the bovine PCR is applied on DNA extracted with the DNeasy(TM) Tissue kit. A PCR of 30 cycles reached a sensitivity of 720 somatic cells in the PCR reaction corresponding to 2.9 x 10(4) cells per ml milk. A PCR of 40 cycles could detect 0.72 to 0.144 somatic cells in the PCR reaction corresponding to 29 to 2.9 cells per ml of milk.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available