Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 67, Issue 8, Pages 3753-3755Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.8.3753-3755.2001
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To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from Pediococcus acidilactici for one mismatch and Micrococcus luteus for the perfect match. PCRs showed that the bias was reduced at lower temperatures. An environmental sample was also examined.
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