Journal
BRAIN RESEARCH PROTOCOLS
Volume 8, Issue 1, Pages 25-31Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1385-299X(01)00081-2
Keywords
locus coeruleus; tetrahydrobiopterin; GTP cyclohydrolase I; high-performance liquid chromatography (HPLC); differential oxidation; fluorescence detection
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Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus. (C) 2001 Elsevier Science B V All rights reserved.
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