Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 65, Issue 8, Pages 1883-1885Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.65.1883
Keywords
cucumber; chitinase; root; xylem sap
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A chitinase activity was detected in fractions of xylem sap collected from the cut surface of cucumber stems. A 28-kDa acidic protein was purified from the active fractions and its N-terminal amino acid sequence was found to be identical to that of a chitinase gene. Cucumber roots produce and secrete an acidic chitinase, one of the PR proteins, into xylem sap and deliver it to aboveground organs.
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