Journal
NATURE IMMUNOLOGY
Volume 2, Issue 8, Pages 718-724Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/90659
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- NIAID NIH HHS [AI 20069] Funding Source: Medline
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Estrogen is a negative regulator of lymphopoiesis and provides an experimental tool for probing relationships between lymphocyte precursors and stem cells. We found that expression of lymphocyte-associated genes and immunoglobulin (Ig) gene rearrangement occurred before CD45R acquisition. Lymphoid-restricted progenitors that were Lin-IL-7R alpha (+)c-kit(lo)TdT(+) (lineage marker-, interleukin receptor 7(alpha+), c-kit(lo) and terminal deoxynucleotidyl transferase(+)) were selectively depleted in estrogen-treated mice; within a less differentiated Lin-c-kit(hi) fraction, functional precursors of B and T, but not myeloid, cells were also selectively depleted. TdT and an Ig heavy chain transgene were detected within a hormone-regulated Lin-c-kit(hi)Sca-I(+)CD27(+)Flk-2(+)IL-7R alpha (-) subset of this multipotential progenitor population. Identification of these extremely early lymphoid precursors should facilitate investigation of the molecular mechanisms that control lineage-fate decisions in hematopoiesis.
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