Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 20, Issue 4, Pages 379-384Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1011206131623
Keywords
amino acid type-selective experiments; automated assignment; HSQC; proteins; triple-resonance
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Four novel amino acid type-selective triple resonance experiments to identify the backbone amino proton and nitrogen resonances of Arg and Lys and of their sequential neighbors in (C-13,N-15)-labeled proteins are presented: the R(i+1)-HSQC and R(i,i+1)-HSQC select signals originating from Arg side chains, the K(i+1)-HSQC and K(i,i+1)-HSQC select signals originating from Lys side chains. The selection is based on exploiting the characteristic chemical shifts of a pair of carbon atoms in Arg and Lys side chains using selective 90 degrees pulses. The new experiments are recorded as two-dimensional H-1-N-15-correlations and their performance is demonstrated with the application to a protein domain of 83 amino acids.
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